|
KMID : 0525720190240020075
|
|
Journal of Chitin and Chitosan
2019 Volume.24 No. 2 p.75 ~ p.83
|
|
|
The Immune Response of Ginsenoside Rh4 on HaCaT Cell Using Microarray
|
|
Rhee Jin-Hui
Kim Jung-Min
Bang1 In-Seok
|
|
|
Abstract
|
|
|
|
We investigated the activities, gene expression patterns, and action mechanisms of ginsenoside Rh4 from HaCaT human dermal keratinocyte treated with minor ginsenoside Rh4, a glycoside of Panax ginseng C. A. Meyer. The treatment with ginsenoside Rh4 did not affect the cytotoxicity and proliferation of HaCaT cells. From microarray analysis, the genes related to cell division, DNA repair, cell cycle, cellular response to DNA damage stimulus, positive regulation of GTPase activity, and immune response were found to be 2-fold up-regulated genes in the HaCaT cells following ginsenoside Rh4 treatment, whereas genes involved in negative regulation of inflammatory response, regulation of cell migration, cellular response to cAMP, and regulation of autophagy were found to be 2-fold down-regulated. Especially, the genes related to the immune responses were predicted to be regulated by the transcription factors ATF1 and TCF12. NFκB and TNF were selected as the upstream regulators of ATF1 and TCF12. The activity of the predicted immune response genes, and the activity of up-regulators (NFκB and TNF), were verified by qPCR. The gene activity of ATF1 and TCF12 increased more than 2 times in HaCaT cells treated with ginsenoside Rh4 for 24 hours. Meanwhile, only the transcription factor NFκB was activated by the ginsenosides Rh4. Therefore, the mechanism of the immune response by ginsenoside Rh4 was confirmed that increased the expression of the related gene from the regulation of ATF1 and TCF12 by the up regulator NFκB.
|
|
|
KEYWORD
|
|
|
DNA microarray, Ginsenoside Rh4, Immune response, HaCaT cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|